The course has remained largely unchanged since the London Marathon was first held in 1981.
Starting in Greenwich Park in Blackheath, runners will pass the Cutty Sark around six miles in before going over Tower Bridge and through the tower blocks of Canary Wharf.
They will then go back through the city and along Victoria Embankment by the Palace of Westminster before ending up on The Mall.
Spectators can watch the action from pretty much anywhere along the 26.2-mile stretch, but some viewing points are better than others.
Those at The Highway will see the runners go past twice while Tower Bridge will provide spectators with the chance to see them cross the halfway point.
The green space near the Cutty Sark maritime museum will offer riverside views as the runners pass by, while those wanting to take in the final stretch can do so at St James’ Park.
For spectators with disabilities, there are accessible viewing points at the Cutty Sark, Canary Wharf, Rainbow Row, Tower Hill and Victoria Embankment.
A full interactive map of the London Marathon route can be found here.
